The Two Frontiers Project

The Two Frontiers Project

Sampling, Sequencing & Culturing

Field-to-data services for researchers, institutions, and industry partners who need environmental genomics done right.

01

Environmental Sampling

We design and execute field sampling campaigns for marine, terrestrial, and built environments. Services include protocol development, field kit preparation, site-specific logistics, and chain-of-custody documentation. We specialize in low-biomass and extreme environments where standard commercial protocols produce inadequate results.

Marine water and sediment
Coral tissue and mucus
Soil and rhizosphere
Air and surface swabs
Biofilm and mat communities
Cryogenic preservation and shipping
02

DNA Sequencing

From targeted amplicon surveys to deep shotgun metagenomics, we handle library preparation, sequencing, and primary data processing in-house. All workflows are optimized for environmental samples with low host contamination and high microbial diversity.

16S / 18S / ITS amplicon sequencing
Shotgun metagenomics (shallow and deep)
Metatranscriptomics
Long-read sequencing (PacBio / Nanopore)
Low-biomass extraction protocols
QC, demultiplexing, and primary assembly
03

Microbial Culturing

We culture and characterize environmental microorganisms from complex samples, including organisms from extreme environments that require non-standard growth conditions. Services range from basic enrichment and isolation through to full phenotypic characterization and cryopreservation.

Anaerobic and microaerophilic culture
High-temperature and low-pH conditions
Selective enrichment from environmental matrices
Pure culture isolation and verification
Phenotypic and genotypic characterization
Long-term cryopreservation and culture banking

Get In Touch

Request services directly

Reach out directly and our team will help scope sampling, sequencing, or culturing work for your project.

Free and open for academic and research use.